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Plates were sealed and placed into a controlled growth chamber for 7 days under the same conditions as mentioned above

Many studies also rely on single or limited time-point sampling, pharmacological inhibition with pleiotropic effects, or global genetic models, all of which further constrain interpretation of temporal sequence and cell-specific causality

The sample preparation is based on protein precipitation and derivatisation followed by HPLC determination with fluorescence detection

It is a critical regulator of antioxidant activity, detoxification, and immune function

Selenoprotein P expression is controlled through interaction of the coactivator PGC-1 with FoxO1a and hepatocyte nuclear factor 4 transcription factors