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Confocal images were for above described applications were acquired with a DMI6000 microscope (Leica Biosystems, Wetzlar, Germany) and analyzed using ImageJ default plugins for co-localization and staining intensity

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First of all, there may be difference in the intensity of ROS formation by paraquat and/or in ROS suppression by antioxidant, between in vitro cell line and cells in physiologic state