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The first direction focuses on reducing HSV-1s infectivity and toxicity towards normal cells by limiting viral replication and assembly ( Figure 1 2 Silencing genes 2.1 Gene 134.5 To enhance the selectivity of HSV-1 for infecting epithelial-derived malignancies while minimizing the risk of infection in healthy somatic cells and preventing uncontrolled spread of HSV-1 to normal somatic cells, numerous research groups knockout the 134.5 gene