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The M1 (CD86 + ) to M2 (CD206 + ) ratio of hPBMC-macrophages was analyzed by flow cytometry using CD45-APC/CY 7 (2D1, 368516), CD68-BV421 (Y1/82 A, 333827), CD86-PE (BU63, 374206), and CD206-APC (15-2, 321110) antibodies (BioLegend, USA)

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For FY 2022 and subsequent years, we subtract the hematopoietic stem cell acquisition charges from the total charges on each transplant bill that showed hematopoietic stem cell acquisition charges before computing the average cost for each MS-DRG and before eliminating statistical outliers

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