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After that, the culture medium was discarded, and the cells were washed with PBS three times, which were further treated with fresh culture medium dispersing FITC-labeled ZMTP nanosheets (40 ppm)

doi: 10.3390/jpm12030458 67 DoanH

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Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 340 nm at 25C Precision pipettes to deliver 2 L to 1 mL volumes Multi-channel pipettes to deliver 20 L to 200 L volumes Tubes to prepare sample dilutions 15 mL conical tubes Incubator at 25C Deionized water Assay Procedure Summary Prepare Working Solution

The half-life of ETV6 was significantly prolonged in FBXW7 KO Ishikawa and HEC-1A cells compared to control cells (Figure S2A-D)