Abolaji AO, Ojo M, Afolabi TT, Arowoogun MD, Nwawolor D, Farombi EO
Protein synthesis is associated a with high energy cost and limits nutrient availability for growth and maintenance of body mass
Parasite pellets were resuspended in buffer (3.5 mM MgCl 2 , 110 mM KCl, 40 mM NaCl, 20 mM HEPES, 6 mM EDTA, pH 7.4) with protease inhibitors (0.01 mg of leupeptin A, 0.001 mg of pepstatin A, 0.35 mg of PMSF) lysed by three freeze/thaw cycles (liquid nitrogen and 37C water bath), and protein content was determined using Bio-Rad DC Protein Assay
Allow vial contents to reach room temperature before reconstitution
We recommend sticking to a capsule daily for about two or three months for optimum results