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Moreover, the possibility that AITC in planta can interact with oxidized glutathione -as it was shown in vitro 77 - in addition to the reduced form also needs to be investigated

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The cells were lysed in 25 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1% -mercaptoethanol, 10 mM MgCl 2 , 2.1 mM leupeptin, 1.53 mM aprotinin and 50 U of benzonase (Novagen)