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The production of ROS in bacteria was determined by flow cytometry

Briefly, the process proceeds with the mRNA synthesis of seleno-GPxs by transcription factors followed by in situ synthesis of Sec on a specific set of tRNA designated as tRNA [ser]Sec and its incorporation into the peptide sequence against the termination codon UGA within ribosomal machinery (Fig

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The expected 18 O incorporation ratio for stochastic incorporation of 47.4% was calculated from the H 2 18 O content before addition of Pt GSTF8