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In brief, cells were harvested and washed twice with PBS, then stained with ROS Deep Red staining solution in a 37 C/5% CO 2 incubator for 30 min

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In a hypoxic and glucose-deprived TME, The metabolism of Gln is essential for tumor cells to survive and multiply ( Additionally, metabolomic analyses reveal low levels of Gln and elevated levels of glutamate in TNBC, suggesting that TNBC is more dependent on Gln and more sensitive to therapies targeting Gln breakdown compared to other breast cancer subtypes ( The role of Gln is critical in both the proliferation and metastasis of cancer cells

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