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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C

This network precisely drives the progress of each stage of biofilm formation and affects its drug resistance through the cooperative interaction, dynamic regulation, and specific signal integration of core transcription factors

Subsequently, these consequences contribute to an increased likelihood of morbidity and mortality among neonates

10.1016/s0140-6736(00)02490-9 Lancet (London, England)

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