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From the several sites that can generate superoxide in the mitochondrial matrix, only the superoxide produced at complex III appears to be released both into the matrix and the IMS (Quinlan et al., 2013)

Sugino N, Karube-Harada A, Taketani T, Sakata A, Nakamura Y

The COA reports HPLC purity (99%), mass spectrometry identity confirmation, visual appearance, residual solvents, and moisture content for the exact lot shipped

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