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They were housed under controlled laboratory conditions with a 12h/12 h light/dark cycle, at a constant temperature of 25C 1C and relative humidity of 60% 10%

Existing clinical studies are often small and heterogeneous, limiting conclusions about standardized dosing, long-term safety, and real-world efficacy

Solid laser (488 nm) was used as excitation source for FAM-labeled probe and a 495545 nm bandpass filter was used for fluorescence detection

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Following manufacturer guidelines for your specific product makes sense given this variability