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Following washing, cortices were mechanically dissociated with repeated mixing using a 5 mL serological pipette and the resulting cell-suspension diluted with Opti-MEM (Life Technologies) supplemented with glucose (20 mM) and Antimicrobial-Antifungal to achieve a final tissue concentration of 0.14 mouse cortical hemispheres

Piezo1 activation suppresses bone marrow adipogenesis to prevent osteoporosis by inhibiting a mechanoinflammatory autocrine loop

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Glutathione metabolism in type 2 diabetes and its role in oxidative stress