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51 Such a restriction, in conjunction with other zoning restrictions, reasoned the Court, "would constitute a nearly complete ban on the communication of truthful information about smokeless tobacco and cigars to adult consumers." 52 The Court found that the restrictions on outdoor advertising of cigars and smokeless tobacco were too broad in that they prohibited advertising "in a substantial portion of the major metropolitan areas of Massachusetts," included oral communications, and imposed burdens on retailers with limited advertising budgets

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Genomic regions containing polymorphisms that differentiated the nearly isogenic lines were identified and corresponding SNP markers of interest were converted to Kompetitive Allele Specific PCR (KASP) markers [63] by LGC Genomics (Beverly, MA) (Additional file 11)