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In the study, researchers administered 300mcg of GHRH or placebo to both young and elderly male subjects and recorded their sleep

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Cells were transfected with AAV shuttle vector (7 g per 15 cm dish), Rep/Cap plasmids (7 g per 15 cm dish) and F6 helper plasmids (20 g per 15 cm dish) using polyethyleneimine

Materials and Methods Organisms and growth conditions C