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Briefly, thiols were derivatized with 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate, and chromatography was carried out on a C18 reversed-phase column with a mobile phase of 0.1 M potassium phosphate buffer (pH 2.1) with 5 % (vol/vol) acetonitrile

Example Protocol Using the HisLink Resin to Purify Proteins from Cleared Lysate by Gravity-Flow Column Chromatography Materials Required: HisLink Protein Purification Resin (Cat.# V8821) and protocol HEPES buffer (pH 7.5) imidazole HisLink Binding Buffer HisLink Wash Buffer HisLink Elution Buffer column Cell Lysis: Cells may be lysed using any number of methods including sonication, French press, bead milling, treatment with lytic enzymes (e.g., lysozyme) or use of a commercially available cell lysis reagent such as the FastBreak Cell Lysis Reagent (Cat.# V8571)

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{Mullineaux PM, Creissen GP