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10.1155/2024/1239661 299 ShuklaS

Materials and methods Cell lines and reagents The MCF-7 human breast cancer cell line was obtained from Dr Dean Edwards (University of Texas, San Antonio, TX, USA) and was maintained in phenol red RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/mL penicillin, 100 g/mL streptomycin, 1 non-essential amino acids and bovine insulin at 6 ng/mL

APAP induced hepatotoxicity in patients is believed to be affected by alcohol intake (ethanol, EToH) with some even reporting death due to this liver injury (Lesser, Vietti, and Clark 1986