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The cDNA was amplified with SYBR QPCR mixture (Yeasen Bio, Shanghai, China) on the BIO-RAD CFX Connect Real-time System (BioRad, CA, United States)

The appropriate dose of CuNPs to be used in vivo was calculated as 1/10 of the LD 50 value (LD 50 = 6.67 mg/kg body weight)

The formula is vegan, fragrance-free, and free from parabens and sulfates, safe, effective, and easy to use every day.

In addition to the cellular changes, 7.5- and 9-month-old animals showed reduced use of hippocampus-dependent strategies, especially in the re-learning phase

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