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Introduction In 1934, Whipple, Minot and Murphy were awarded the Nobel Prize for their game-changing discovery that feeding liver meat to patients affected by pernicious anaemia could act as a curative therapy for this previously invariably fatal condition ( Today, Vitamin B12 deficiency is a commonly encountered clinical diagnosis and most clinicians will have prescribed B12 replacement therapy for some of their patients
NPPCs are able to differentiate into the chondrogenic lineage in vitro , and to survive in the aneural, avascular, hypoxic NP [10, 14]
It works well for doses of 2.5 mg and above, where 25 units or more are being drawn
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These frameworks enable first engineering, validated through SPR and in vitro assays, and offer scalable solutions to overcome limitations in dataset size, structural modeling, and experimental throughput
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