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Tuning local calcium availability: cell-type-specific immobile calcium buffer capacity in hippocampal neurons

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For compound treatment, the medium was replaced by 90% of Williams E Medium supplemented with 12.5% HepaRG preinduction and toxicity medium supplement, 0.6% HepaRG induction medium supplement (Lonza, catalog: MHIND), and 25 U/mL penicillin-25 g/mL streptomycin

Its the only way to ensure that the potential held within that small vial can be accurately and reliably explored

Conservative approaches use shorter 4-6 week cycles with 2-4 week breaks, repeating as needed