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p53 TAD (13-61) was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3) pLysS cells

Treatment Treatment includes Positional therapy, weight loss, oral appliances, continuous positive airway pressure (CPAP), treatment of nasal allergies, exercise and surgery (Sia et al., 2017)