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Though DAM has been detected across multiple neurological disease models, the composition of disease related microglia and macrophage clusters differs between diseasephagocytic subtype predominant in AD, while inflammatory subtype predominant in ALS (Wishart et al., 2023)

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Using HEK-293 adherent cells and K-562 suspension cells treated with varying concentrations of menadione or l-Buthionine Sulfoximine (BSO), two compounds known to affect total glutathione (GSH + GSSG) levels, we assayed the cells in separate assays and in a multiplex assay

For example, CRISPR-Cas9 gene editing technology can be used to edit genes related to ROS generation and clearance, such as NQO1, p53, and NRF2, thereby regulating ROS levels [513,514,515]