J Basic Clin Physiol Pharmacol
On average they found that water inside bottles used by adults went from having around 75,000 bacteria per millilitre in mid-morning to more 1-2 million per millilitre over the course of 24 hours
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Mousavi et al., 2013
Using the wrong solution type is another frequent error: substituting tap water, saline, or an unverified liquid for pharmaceutical-grade bacteriostatic or sterile water introduces contamination risk regardless of how carefully everything else is handled
