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Quantitative Reverse Transcriptase Polymerase Chain Reaction Analysis One hundred milligrams of kidney tissue were homogenized in TRIzol reagent (Servicebio Technology Co., Ltd., Wuhan, China) to extract total RNA, and 1 mg of RNA was reverse transcribed using a RevertAid First Strand cDNA Synthesis Kit (#K1622, Thermo Fischer Scientific)

2 Modifications of chitosan structure/chemistry of chitosan and its derivatives Chitosan is chemically modified to enhance its ability to dissolve, rheological features, thermal integrity, and oxidation resilience (Huang et al., 2018)

Hum Reprod Update 20:517529

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