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While they are normally the result of stress, free radicals can be caused by other environmental factors like smoke and pollutants

Soybean nodules contain ~4-fold more homoglutathione than glutathione, while the roots contain nearly 80-fold more homoglutathione (Matamoros et al., 1999)

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and with chronic use of ibuprofen for 4 weeks, 30 min after last application

Next, we treated HepG2 cells with BCNU, a chemical inhibitor of GR, the enzyme converting GSSG back into GSH