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Under the stimulation of growth factors, PI3Ks are recruited onto the plasma membrane to catalyze the phosphorylation of the 3-hydroxy group of PIP2 to generate PIP3, which then acts as the second messenger, recruiting PDK1 and AKT proteins onto the plasma membrane to partially activate AKT by PDK1

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and others, whose experiments also revealed increased activity of Sox9 and aggrecan when stimulated by PRP

Nitric oxide (NO) is a small signaling molecule involved in how cells communicate, especially in contexts linked to vascular biology and cellular stress responses