Five microliters of cell concentrations (10 5 , 10 4 , 10 3 and 10 2 ) of each yeast culture were spotted onto YPD plates treated or not with H 2 O 2 4 mM, in presence or in absence of the GSH precursors (0,02 M glutamate, 0,02 M cysteine, 0,02 M, glycine) or with BSO (L- buthionine-sulfoximine) 5 mM
Gaffney, E
Also, the officer will issue a temporary license for 30 days
For practitioner dispensing only.
After 4 hours incubation, the infected cells were probed for mature virus with A12 primary antibody (anti-PV human serum), which binds specifically to mature virus and provirions (Nihal Altan Bonnet, unpublished results), and Alexfluor 488-conjugated secondary antibody (green color)