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In brief, 0.5 ml of the supernatant of brain homogenate was added to exactly 4.5 ml of working reagent (1 volume from 0.8% thiobarbituric acid + 3 volume from 20% trichloroacetic acid)

Chem Biol 15:234245 Wewer M, Bittker JA, Lewis TA, Shimada K, Yang WS, MacPherson L et al (2012) Development of small-molecule probes that selectively kill cells induced to express mutant RAS

Although some studies have found that the bacterial load of plastic bottles can be higher than those of stainless steel bottles, the cleaning strategy used tends to make a far bigger difference

NNAL, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol

The longer, the better so that more gelatin and nutrients are released into the liquid