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All real-time PCRs were performed three times and all changes in gene expression were reported as multiples of increases relative to the control while GAPDH was used as an internal control and relative gene expression between the experimental groups were determined using MxPro software

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When the availability of glycine is lower than necessary for sufficient glutathione synthesis, the initial molecule of cysteine and glutamate converts into a compound that is excreted

The metabolic and pharmacokinetic differences between species are relevant, and Phase 1 human safety trials have not been completed for BPC-157