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The seeding medium consisted of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122)
[Google Scholar] 70.Marks AR, Harley K, Bradman A, et al
During the conversion of isocitrate to -KG, Idh1 catalyzed the generation of NADPH, and NADPH and reduced nicotinamide adenine dinucleotide (NADH) were reductants for lipid biosynthesis and antioxidants that protect cells from oxidative stress and radiation damage [77]
The formation of ROS in the vasculature is catalyzed by several enzymes such as NADPH oxidase and uncoupled NO synthase (NOS)