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Cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS, L-glutamine, penicillin, and streptomycin at 37 C in 5% CO 2

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The three compounds, in a rigorous research approach, require: strict analytical control (MS + HPLC 98%), adapted administration route (intranasal for BBB crossing), standardised behavioural model with positive control, and exhaustive documentation for publication

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