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Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

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Moreover, an increased recruitment of adipocytes to the wound site further supports simultaneous tissue regeneration 37

If you are afraid of needles, inform your doctor before the shot as he will take necessary precautions to help you during the procedure and keep you calm

For long-term preservation, freezing at -20C is recommended