PMID: 39060389 Acta Pharm Sin B 2021 Dec;11(12):4045-4054
The plasma level of anti-Tat IgG1 (typical of Th2 response) as well as the levels of anti-Tat IgG2a and IgG2b measured by ELISA assay were lowered and increased, respectively, among Tat-immunized mice pretreated with GSH-C4 or I-152, implying the Th1 response [66,70,91]
Moreover, the majority of these technologies remain in the preclinical phase, lacking validation in human clinical trials or demonstration of scalable manufacturing processes
Proper peptide handling and storage may help reduce degradation pathways such as hydrolysis, oxidation, or structural destabilization during experimental use
For the invasion assay, the membrane of the transwell unit was coated with 35 l Matrigel at 37C for 4 h to form a reconstructed basement membrane