D., Tarlinton, D
Potential Risks Though rare, some risks are possible, especially if glutathione IV therapy is not administered properly
Astrocytes were cultured in DMEM/F-12 media containing HEPES (10 mM), sodium bicarbonate (13 mM, pH 7), 10% fetal bovine serum, penicillin and streptomycin (100 g/ml each, invitrogen) as described [30]
Cells were then stained with secondary antibodies (goat anti-mouse IgG H&L (Alexa Fluor 647), Abcam, ab150115-1001 and goat anti-rabbit IgG H&L (Alexa Fluor 488), Abcam, ab150077-1001) at 1:1,000 in BK buffer at room temperature for 30 min, followed by staining with DAPI at 1:10,000 in PBS
TGF-1 could also induce adult mouse hepatocytes to undergo EMT, and up to 45% of the FSP1-postive fibroblasts are derived from hepatocytes in carbon tetrachloride (CCL4)-induced fibrotic liver (94)