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Mice were given 300 mg/kg APAP (Cayman, Michigan, USA) to induce liver injury or saline for control following an overnight fast

In further experiments, four insomniac patients adapted in the laboratory for 1 night, with placebo injections for 2 nights, were treated continuously for 4 days with DSIP administered 1 hour before bedtime

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In this way, the signal travels within the nerve cell until it reaches the synapse, more precisely its element called the presynaptic ending