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Researchers do this to equalise pressure, but it introduces airborne contaminants and creates a positive pressure differential that forces solution back through the needle tract on every subsequent draw, accelerating bacterial contamination

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Stimulation of GSH synthesis to prevent oxidative stress-induced apoptosis by hydroxytyrosol in human retinal pigment epithelial cells: activation of Nrf2 and JNK-p62/SQSTM1 pathways