[DOI] [PMC free article] [PubMed] [Google Scholar] Lin X, Tascilar M, Lee WH, Vles WJ, Lee BH, Veeraswamy R, Asgari K, Freije D, van Rees B, Gage WR, Bova GS, Isaacs WB, Brooks JD, DeWeese TL, De Marzo AM and Nelson WG
Injectable anti-aging protocol: Dose: 1-2mg daily Frequency: 5 days on, 2 days off OR daily for 30 days Cycle: 30 days on, 14 days off Duration: Minimum 2-3 cycles for substantial results Topical anti-aging protocol: Concentration: 2-3% serum or cream Application: Twice daily (morning and evening) Duration: 12+ weeks continuous use Combine with: Sunscreen (always), retinoids (evening, separate application) Combination approach: Maximum anti-aging results come from combining injectable and topical
Dosage and frequency vary based on individual health status and goals
Bioinformatics 36 , 925927 (2020)
For cryosections, pancreases were fixed in 4% paraformaldehyde (PFA), cryoprotected in 30% sucrose, embedded in optimal cutting temperature (OCT) compound and sectioned at 5 m