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J Physiol Paris (2011) 105:1839.10.1016/j.jphysparis.2011.07.002 182 MilhietVBoudebesseCBellivierFDrouotXHenryCLeboyerMet alCircadian abnormalities as markers of susceptibility in bipolar disorders

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Do not use phosphate-buffered saline (PBS) at high concentrations, as phosphate can compete with the peptide for copper coordination at elevated temperatures, potentially displacing the metal