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Samples were frozen at 80 C until use

It is now apparent that the production of primary immune mediators, which include cytokines and chemokines, is dependent on the recruitment of inflammatory cells, particularly innate immune cells such as neutrophils, macrophages, and DCs

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Pseudomonas syringae , a terrestrial bacterium, produces a protein which is the most efficient known biological INP, with an onset freezing temperature of 1.8 C and completely activated by 12 C 45