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These LEDs were selected to match the Soret band (405 nm), the shortest (500 nm), and the longest wavelength (630 nm) Q bands of porphyrin absorption spectrum and a spectral range (460 nm) where the porphyrin absorption spectrum has a minimum

Dimethyl sulfoxide diluted in a .01 M glycine buffer was added to solubilize the cell membranes and the absorbance of reduced MTT in the buffer was quantitated at 590 nm using a plate reader (Biotek Synergy 2, Winooski, VT)