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doi: 10.1016/j.cub.2014.03.034

After treatment with the blocking solution (0.3% Triton-X and 3% BSA in 0.01 M PBS) to block non-specific staining and increase permeability to antibodies, the brain sections were incubated with anti-c-Fos rabbit polyclonal antibodies (abcam) in the blocking solution at 4 C overnight

Second, COP1 recognizes substrates via its WD40 domain and engages DET1 through asymmetric WD40-DET1 interfaces, consistent with a COP1 dimer bound to a single DET1

Lmna(K32) knock-in mouse model exhibited incomplete tissue maturation and severe metabolic defects, ultimately leading to premature death 36