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It also increases integrin expression and the transcription factor p63, both of which support the proliferative and synthetic activity of dermal fibroblasts

ROS generation and cell apoptosis To better understand the mechanism of cell death induced by NPs in senescent chondrocytes, we first measured total intracellular ROS generation via a DCFH-DA method

The organic phase was collected, dried in the vacuum concentrator and redissolved in 53 l of isopropanol, centrifuged and 50 l were transferred in glass vials for LCMS analysis