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These derivatives comprise three major modifications of MB (Figure 1): 1) side chain deletions at positions 3 and 7 (phenothiazine and 2-chlorophenothiazine), 2) substitution of a side chain at position 10 (chlorpromazine and promethazine), 3) substitution of sulfur at position 5 with nitrogen (neutral red)

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In adipocytes, NNMT inhibition elevates NAD and SAM and suppresses de novo lipogenesis in a concentration-dependent manner, consistent with on-target activity [13]