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doi:10.3109/15563650.2013.802797 10 Treweeke, A

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After 30min of further incubation, the conversion of DCFH-DA to the fluorescent product DCF was measured by a confocal laser scanning microscope with excitation at 488nm and an emission at 525nm to determine the concentration of ROS in the samples

Once prepared, it is administered subcutaneously (under the skin) using a fine insulin syringe