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Approximately 150 g of digested peptide from each sample was used for purification, which is within the C18 spin columns maximum binding capacity of 100 g (#744101, Harvard Apparatus, Holliston, MA, USA) and then lyophilized before being analyzed on the Orbitrap Fusion Tribrid mass spectrometer coupled with an Ultimate 3000 nano-UPLC system (Thermo Fisher Scientific, Waltham, MA, USA)

Abstract Neurodegenerative diseases are characterized by progressive neuronal dysfunction, often accompanied by chronic inflammatory states and redox imbalance within the central nervous system (CNS)

This integrative role connects it to chronobiology research rather than isolated sleep studies

Saw Palmetto is a natural plant extract famous for boosting hair growth

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