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Timing matters here

In severe cases, intravenous (IV) fluids containing electrolytes can provide rapid hydration and symptom relief

It's also a good idea to avoid mixing copper peptides with strong oxidizing acne treatments like benzoyl peroxide in the same step, as this can exacerbate skin irritation and compromise peptide stability

However, the inevitable tissue disruption on extraction into aqueous buffer has the potential to generate artifacts arising from the interaction between a GST and a ligand usually found in a different compartment, or the generation of ligands formed through chemical- or enzyme-mediated oxidation during extraction

The cost of replacing degraded peptides typically exceeds the minimal effort required for appropriate temperature control