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Weiss GA, Hennet T (2017) Mechanisms and consequences of intestinal dysbiosis

Mammalian cell culture medium was centrifuged twice at 12,000 g for 20 min at 4 C, later the supernatant pH was adjusted to 8.0 with equilibration buffer (50 mM sodium phosphate, 300 mM NaCl and 20 mM imidazole, pH 8.0)

JL: Conceptualization, Investigation, Writing original draft

761 The previously noted strong evolutionary similarity of bacterial ZigA/ZagA to the mycobacterial Mrf (MPY recruitment factor) deserves comment