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Owing to its broad and potent activity profile and high safety margin in mammals, GHK-Cu is a valuable tool for studying tissue repair, regenerative biology, age-related degeneration, and as a model therapeutic agent in peptide research 1,2,4

*Correspondence: Zhenjie Liu, [email protected] These authors have contributed equally to this work and share first authorship This article was submitted to Biosafety and Biosecurity, a section of the journal Frontiers in Bioengineering and Biotechnology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference

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The coverslips were placed facing the droplets and incubated overnight at 4C