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The best binding was observed for the histidine containing peptide as expected, whereas slightly weaker affinity was observed for amide (Q, N) or amine (K) containing side chain, whereas carboxy (D,E), thioether (M) or aromatic (F,Y,W) functional groups hardly enhanced affinity compared to the single histidine containing peptide (Fig

Relevant roles of each specific IGFBPs will be further discussed when applicable to metabolism since there is a huge emerging world of IGFBPs independent actions

These findings highlight the significance of small moleculemacromolecule interactions in the functional development of natural compounds and underscore the therapeutic potential of flavonoids in cardiovascular disease modulation and health maintenance