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Important Warnings

Mechanisms of lactylation in cancer Enzymes and pathways involved in lactylation The mechanisms of lactylation can be broadly categorized into enzymatic and non-enzymatic pathways

The bound proteins were eluted by boiling with SDS-PAGE sample buffer without DTT for 10 min at 95 C, and then analyzed by immunoblotting

The homogenate was centrifuged at 900 g for 5 min at 4 C, and the supernatant was split into three equal parts, transferred to a new tube, and centrifuged at 20,000 g for 20 min at 4 C to collect membranes

Although alcohol, in moderation, is not necessarily deleterious, and may even have some positive benefits, more recent studies recommend that alcohol should not be consumed for its potential health benefits